动物模型,门静脉高压 z-bo 2020-08-06 554

　　(1) Reproduction method Male Wistar rats weighing 150-200 g were intraperitoneally injected with 3% thioacetamide (TAA) at a dose of 50 mg/kg body weight, once a day for 16 weeks. Two weeks after TAA administration, the hepatocyte eosin staining around the central vein can be seen deepened; after 4 weeks, some liver cell necrosis can be seen in the center of the lobule, lymphocytes and neutrophils can be seen around it, and the whole lobule liver cells and nucleoli have increased Large, yellow-brown sedimentary macrophages formed by phagocytic necrotic hepatocytes can be seen around the central vein; fibrous cords can be seen after 8 weeks; typical liver cirrhosis appears at the end of 10 weeks, and different degrees of liver cell dysplasia can be seen. However, the mortality rate of intraperitoneal injection is relatively high, ranging from 5% to 28%, and the molding rate is 75% to 100%.

　　(2) Model features The model has a high success rate, relatively short modeling time, simple modeling methods and easy operation.

　　(3) Comparative medicine Thioacetamide (TAA) is a hepatocellular toxic substance, which can reduce the content of P450 in the liver cell nucleus, inhibit respiratory metabolism and enzyme activity in the liver cell nucleus, and increase liver cell DNA synthesis and mitosis. Form cirrhosis of the liver. The liver cirrhosis model made by this method has histological changes similar to human cirrhosis, and the portal vein pressure is significantly increased and a certain degree of portosystemic tributary shunt occurs. Using TAA to make rat liver cirrhosis model is better than CCl4.