动物造模,糖尿病大鼠模型 z-bio 2021-05-28 797

　　Objective: To observe the changes in the structure and abundance of intestinal flora in a rat model of diabetes induced by streptozotocin (STZ).

　　Method: 25 male SD rats were randomly divided into control group (C) 10 and model group (M) 15. Inject 3% STZ intraperitoneally into the model group for 5 consecutive days at a dose of 30 mg/(kg·bw). After building the model, we will measure your weight, blood sugar and other indicators every month. At the 4th and 12th week of establishing the model, fresh rectal feces of each group of rats were collected at the same time. The bacterial 16SDNAV3 region in stool samples was sequenced on Illumina's high-throughput sequencing platform to quantitatively analyze the structure and abundance of intestinal flora.

　　Result: There is no significant difference in the number of optimized sequences between the model group and the control group (P\→0.05). The relative abundance index (Chao1) and diversity index of the intestinal flora in the model group were lower than the control group (P\u003c0.05), and the Simpson index was higher than the control group (P\u003c0.05). u003c0.05).. ). At the phylum level, the relative abundance of Proteus, cyanobacteria, tenericutes, TM7 and actinomycetes was reduced (P\u003c0.05). At the genus level, the relative abundance of Lactobacillus in the model group decreased (P\u003c0.05), while the relative abundance of Bacteroides increased (P\u003c0.05). After 12 weeks of modeling, the relative abundance of Lactobacillus, Bacteroides and Rumencoccus model groups increased (P\u003c0.05); the relative abundance of Bifidobacteria decreased (P). u003c0.05).

　　Conclusion: The decrease in the number and diversity of intestinal flora induced by STZ provides a reference for studying the relationship between diabetes and intestinal flora.