动物造模,高血压模型 z-bio 2021-05-28 436

　　Objective: How to establish a hypertension model of Bama mini-pigs with a high-fat and high-salt diet and explore its possible pathogenesis?

　　Method: 18 Bamamini male pigs were randomly divided into 3 groups: normal control group (NC)? Are there 6 animals in the high-fat (HF) group and the high-fat and high-salt (HFHS) group? Group C received normal diet feeding, HF and HFHS groups received high-fat diet, high-fat diet and high-salt diet for 24 consecutive weeks respectively? The systolic blood pressure (SBP) and diastolic blood pressure (DBP) of the minipigs were measured at 8, 16, and 24 weeks. Within 24 weeks of modeling, blood glucose, blood lipids, liver and kidney function, and plasma endothelin 1 (ET-) were measured. 1) Take renin (renin), angiotensin II (AngII), aquaporin 2 (AQP-2), vasopressin (AVP) and vascular endothelial growth factor (VEGF) and other indicators, and the liver? Histopathological observation of the kidney?

　　Results: The NC group, HF group and HFHS group showed a significant increase after 8 weeks of comparing the model with SBP and DBP, showing a continuous upward trend. The HFHS group was higher than the HF group; at the same time, the model was one week after 24 weeks , The body weight of miniature pigs and the liver and kidney indexes of HF and HFHS groups were significantly increased (P\u003c0.05), and plasma TC, CREA and ET-1 levels were also significantly increased (P\u003c0). .05, P\u003c0.01). On the other hand, the BUN level of the HFHS group increased significantly. Decrease (P\u003c0.05), but renin? expensive? Me AQP-2? Is the AVP content significantly increased (P\u003c0.05, P\u003c0.01)? Oil red "O" staining results indicate liver lipid deposition. Pathogenic changes, such as kidney and renal artery thickening in HF and HFHS groups?

　　Conclusion: A high-fat and high-salt diet can be established 8 weeks after induction to establish a mini-pig hypertension model. The pathogenesis may be related to affecting renal function, activating the RAS system and AVP?