动物模式,门静脉高压 z-bo 2020-08-06 558

　　(1) Reproduction method Male Wistar rats weighing 200～250g are anesthetized by intraperitoneal injection of sodium pentobarbital at a dose of 30mg/kg body weight. The abdominal midline is incised in each layer of the abdominal wall, the common bile duct is separated, and the bile duct is separated from the middle after double-line ligation. The common bile duct was cut, and 30 weeks later, the portal vein was intubated to measure the pressure. Experimental results showed that the portal vein pressure increased significantly after surgery.

　　(2) Characteristics of the model In this experiment, samples were taken for pressure measurement 4 weeks after the operation. The liver was still in the fibrosis stage. Although the portal pressure was significantly increased, there were no typical symptoms of cirrhosis. The degree of portal pressure increase in this model is lower than that of the partial portal vein ligation model. This model is suitable for the study of biliary cirrhosis.

　　(3) In comparative medicine, the common bile duct is ligated and narrowed to block bile drainage, intrahepatic cholestasis, liver tissue gradually degeneration, necrosis, and inflammatory cell infiltration, small bile ducts in the portal area proliferate and gradually expand to the liver lobules, thus forming Bile cirrhosis is a gradual process, which is similar to the pathological process of biliary cirrhosis.