动物造模,幼羊颅骨缺损模型 z-bio 2021-05-31 299

　　Objective: To detect the expression of Dlx2 gene in the skull tissue of a small tail skull defect model, and to explore its importance in skull defects?

　　Method: Choose 10 one-month-old small-tail sheep to establish a skull defect model. Collect native skull tissue adjacent to the defect, and use real-time fluorescent quantitative PCR and Western blotting to detect the mRNA and protein expression levels of Dlx2?

　　Result: Compared with the skull tissue in the native tissue adjacent to the defect site, the new skull tissue Dlx2 mRNA and protein expression levels in the defect site are significantly higher (P\u003c0.05)?

　　Conclusion: Increased Dlx2 gene expression may be important for skull defect repair?