动物造模,肺腺瘤小鼠模型 z-bio 2021-10-26 353

　　Objective: How to establish a mouse lung adenoma model and perform blood biochemistry, blood routine, urine routine, and pathological examinations?

　　Methods: 7 B6.129S4-Krastm4Tyj/J positive mice, 4 experimental groups, 3 PCR identifications to obtain the control In addition, set up 3 wild-type control groups, once a week to the experimental group and wild-type control group in the nasal cavity Cre adenoma virus was instilled, and a total of 4 urine tests, blood biochemistry, biochemistry, blood tests, lung collection, histopathology and fluorescence quantitative PCR were used to detect Cre expression: 4 positive mice had multiple lung adenomas, with an average of 4.25, The volume is (10.81±6.38) mm3. No tumor is found in the control group? Is the tumor swelling and growing? The borders are clear, and the tumor cells are cubic epithelial? Arranged in irregular ducts Yes, there is no thread splitting? Compared with the control group, the lung adenoma mice have higher low-density lipoprotein cholesterol? The average platelet volume is significantly different; is the increase in direct bilirubin compared to the control group lower in HDL cholesterol than the control group? Is the difference important?

　　Conclusion: As a result, a mouse lung tumor model was established, and their blood biochemistry, blood routine, urine routine, and pathology were measured?