动物造模,肢体缺血-再灌注损伤模型 z-bio 2021-05-31 338

　　Objective: To introduce an improved method of blocking and blocking the blood flow of the femoral artery in vitro, and to establish a simple, controllable, and reliable rat model of limb ischemia reperfusion injury (LIRI)?

　　Method: 15 2-month-old SPF rats and SD male rats were randomly divided into 5 groups: normal group, ischemia-reperfusion 0 hour group, 2 hour group, 4 hour group, and 8 hour group. normal group. The blood flow was not blocked, and the other 4 groups were constructed with ischemia-reperfusion injury. Model, use HE staining to observe the morphological changes of skeletal muscle cells, and use DAPI to detect cell apoptosis?

　　Results: In addition to the normal group, the fibrous space of each group had different degrees of muscle fiber swelling, irregular arrangement, muscle expansion, etc. The degree of injury in the 4h and 8h groups was significantly lower than that in the 0h and 2h groups. The 8h group had the most severe damage, and the four groups of skeletal muscle cells all showed typical morphological characteristics.

　　Conclusion: The method is simple and easy to implement. Effective as a clinical feature of LIRI, can LIRI reflect the characteristics of pathological changes in skeletal muscle and be used to establish animal models of limb ischemia-reperfusion?