动物造模,树鼩真菌性角膜炎模型 z-bio 2021-10-11 388

　　OBJECTIVE: To study the changes in the expression of mannose receptors and inflammatory factors on the cornea infected by dietary Fusarium, and to explore the mechanism of fungal infectious keratitis. Tupai mannose receptor qPCR detection during keratitis? Dectin-1 expression? IL-1β? IL-6? TNF-α; Tupai primary corneal cells were cultured in vitro and tested for infection?

　　Results: The success rate of Tupai fungal keratitis model establishment was 84? MR and Dectin-1 were early high copy number positive expression infections, leading to the peak expression of inflammatory factors IL-1β, IL-6, and TNF-α, and the expression of these factors It is positively correlated with the severity of the disease, and there is no difference in the expression of IL-1β. IL-6 and TNF-α are significant before and after corneal cell infection in vitro (P→0.05)?

　　Conclusion: The established tupai model of fungal keratitis has similar pathological symptoms to human fungal keratitis? Mannose receptors are involved in regulating the body's immune response to fungal infections, and the body's own immunity plays a role in the development and outcome of corneal inflammation. Plays an important role.