动物造模,人源性肝癌细胞小鼠原位移植瘤模 z-bio 2021-11-01 766

　　Objective: To select 3 strains of human hepatocellular carcinoma cells and inoculate 4 different types of different immunodeficiency mouse liver tissues in situ to establish an orthotopic transplantation model of human hepatocellular carcinoma cells and compare them?

　　Method: Human HepG2 HUH-7 and QGY-7703 cell suspension were respectively inoculated into the liver tissues of immunodeficient mice (BALB/c nude mice, NODSCID mice, NOG mice, NPG mice), and how long did they take? mortality rate? Liver weight, B-ultrasound and histological detection methods to analyze and compare the characteristics of liver cancer models in different immunodeficiency mice?

　　Results: B-ultrasound examination and gross anatomical observation showed that there were tumor nodules in the liver tissues of all mice in each experimental group. All animals in the liver of each group inoculated with HepG2 cell suspension died about 20 days after the experiment. The survival time of NOG and NPG mice was significantly shorter than that of BALB/c nude mice and NODSCID mice (P). u003c0 .001); Animals inoculated with HUH-7 and QGY-7703 livers in each experimental group were dissected on the 92nd and 104th days of the experiment, reducing the liver volume in the OG and PG models. I found it. Mice increased significantly and formed large tumor masses, but BALB/c nude mice and NODSCID mice showed only small tumor nodules in liver tissue; liver weight of NOG and NPG mice inoculated with HUH-7 and QGY7703 Significantly higher than BALB/c nude mice and NODSCID (Pu003c0.05); histological examination showed that the liver tissues of each group had tumor cell proliferation and extensive necrosis, indicating that tumor cell metastasis occurred in the lung tissue of some animals?

　　Conclusion: Comparing BALB/c nude mice and ODSCID mice, liver cancer cells are OG, and NPG mice are liver tissue. Does it grow faster and eventually show a shorter survival period, larger liver, and weight gain? OG and NPG mice can complete malignant growth. Human liver cancer cells can shorten the model research cycle in a short time. NOG and NPG mouse orthologous transplantation of human liver cancer is an ideal model for the development of anti-liver cancer drugs?