动物造模,薄型子宫内膜小鼠模型 z-bio 2021-12-16 1400

　　Objective: To further explore the possibility of establishing a stable thin endometrial mouse model by injecting 95 tanol into the uterine cavity through immunohistochemistry and fertility assessment. This model is an ideal animal for thin endometrial. Provide relevant research for the pathological characteristics and repair mechanism of thin endometrium.

　　Method: Select 78 sexually mature female C57BL/6J mice as experimental animals, place 95? Tanol in the uterine cavity, and establish a thin endometrial mouse model by injecting the endometrium to damage the endometrium. One of 30 groups, 78 of which The experimental mice were randomly divided into two and randomly blank. The experimental mice were divided into the control group and the experimental group. In the third estrus period after the model was established, the experimental mice were killed by cervical dislocation, and uterine specimens were collected. Immunohistochemistry Detect expression. Analyze cytokeratin, vimentin, vascular endothelial growth factor and estrogen receptor alpha to evaluate endometrial cell regeneration; another group of 48 rats were randomly divided into blank group, control group, unilateral injury group, and divided into double groups Lateral injury group. After modeling, all surviving mice were mated during the third estrus, and the effect of thin endometrium on mouse fertility was analyzed. Thin endometrium mice

　　Results: The results of immunohistochemistry showed that intrauterine injection of estrogen, vimentin and 95 tanol in the experimental group of mice was vascular endothelial growth factor and estrogen receptor alpha was significantly lower than that of the blank group and the control group? The mice were conceived normally, the mice in the bilateral injury experimental group were not pregnant, and the unilateral injury experimental group was uterine injury. Side unconceived. The injured uterus is pregnant normally, and the average pregnancy rate of the injured uterus is significantly lower than that of the uninjured uterus (Pu003c0.05). Does intrauterine injection of 95 Tantanol indicate that it will damage the mouse endometrium and reduce fertility?

　　Conclusion: This study shows that the endometrium is thin. Have you established a thin endometrial model that can be used to study membrane repair and repair mechanisms?