动物造模,果蝇心衰模型 z-bio 2021-10-21 386

　　Objective: To establish a heart failure model of fruit flies induced by a high-fat diet?

　　Method: The wild-type fruit fly w1118 fruit fly was selected as the experimental object, and coconut oil of different concentrations (0?7.5?15?., 30? Food. Do you feed the fruit flies for 5 days to test the triglyceride content of the fruit flies? Follow-up experiment Use the non-high-fat feeding group (0 coconut oil) as the control, and the 30? oil-high-fat feeding group as the coconut experimental group? Negative geotaxis test to detect the locomotor ability of Drosophila? Drosophila adult heart function analysis platform, under the semi-anatomical state of Drosophila (30s) Under the beat of the heartbeat, the adult heart software analyzes and obtains functional indicators including heart rate cycle, systolic blood pressure and systolic blood pressure, diastolic diameter, systolic index, etc.? Real-time quantitative PCR to detect changes in the heart RNA expression of high-fat fruit flies? High-fat fruit Flies have different proportions of triacylglycerol content, and high-fat flies are given coconut oil according to the concentration. Increase (Pu003c0.05); high-fat diet significantly affects the motility of flies (Pu003c0.001); feeding high-fat flies The heartbeat cycle is short (Pu003c0.01), and the heart contractility is weak (Pu003c0.001); real-time quantitative PCR high-fat in the feeding of fruit flies, the expression of several genes is related to lipid metabolism, fa2h, fa2h, CG6277, CG3699 , CG9914 and lip2, but significantly down-regulated (Pu003c0.05)?

　　Conclusion: High-fat diet successfully established a model of adult Drosophila heart failure that severely affects the exercise capacity of fruit flies and high-fat diet-induced adult heart Drosophila heart failure?