动物造模,深静脉血栓模型 z-bio 2021-10-14 312

　　Objective: To observe the expression and significance of thrombomodulin (TM) in the natural evolution of the formation and resolution of deep vein thrombosis (DVT) in rats?

　　Methods: The SD rat model group (n=60) was randomly divided into a control group (=6). The model group adopted the "stenosis method" to establish the DVT model, and the control group adopted the "sham operation method", and the model was established 1h-4h after modeling For? 6h? 12h? 24h. Rats were sacrificed at 10 o'clock on the 28th day from 3d to 7d to 14d to 21d. The thrombus weight/length ratio was measured, the soluble TM concentration in plasma was measured, and the expression of TM mRNA in thrombus tissue and inferior vena cava endothelial cells was measured. .

　　Results: 1) The thrombus weight/length ratio (1?4?6?12h) at the initial stage of modeling was relatively low, and the difference between the four groups was not significant (P→0.05); 24h?3d?7 days after modeling, Stable at a higher level (P→0.05); after 14 days, it starts to decrease, but there is no significant difference from the results of 21-28 days (P→0.05) )? (2) ELISA results show that the plasma solubility of TM is higher than that of the control group. Significant (P0.05)? 4) Thrombus weight/length ratio is negatively correlated with endothelial TM mRNA (r = -0.92, Pu003c0.01)); thrombotic TM mRNA is positively correlated with plasma soluble TM (r=0.96 , Pu003c0.01)?

　　Conclusion: Does the change in endothelial TM mRNA expression reflect the progression of DVT and have a good prognostic effect?