动物造模,结肠癌肝转移裸鼠模型 z-bio 2021-10-13 544

　　Objective: To establish a liver metastasis model of colon cancer with high metastasis rate? easy to use? The results are reliable, and are used in experimental studies to prevent colon cancer metastasis?

　　Method: 15 Balb/c nude mice were divided into 3 groups (group A) (group B, group C), and 5 Balb/c mice were divided into group D. The colon is implanted into the spleen, and the cell concentration is 2.5 x 107/mL. HCT116 CT26 stores the spleen in 0.2 mL of cell suspension. Comparison of the 4 groups of cancer liver metastasis models, the success rate of animal model models and the size of liver metastases? Number and abdominal metastasis?

　　Results: The success rate of nude mice in group A was 100-5/5). The number of liver metastases in the liver were all splenic tumors, which were small and scattered, mostly distributed in the right lobe of the liver, with an average survival rate of (26.6±3.4) days. Nude mice in group B The survival rate is 40? (2/5) and metastases are scattered on the surface of the liver. The volume is larger than that of group A. The average survival time is (36.8±4.2) days. The success rate of nude mice in group C is 100-5/5). The liver and spleen are tumors. There are a large number of liver metastases, and multiple metastases fused to occupy the entire right lobe of the liver. The average survival time was (20.2±2.6) days; no metastasis was found in the liver of group D? Three groups of nude mice (group A 2, group C 3) found abdominal metastases, without heart, lung, brain or kidney metastasis. .. Does the liver metastasis of the three groups of nude mice meet the characteristics of adenocarcinoma?

　　Conclusion: The method of protecting the spleen can achieve a higher success rate of model building. It can effectively simulate the method and process of human colon cancer cell metastasis.