动物造模,左氧氟沙星耐药小鼠模型 z-bio 2021-10-07 378

　　Objective: To detect the effect of low concentration of levofloxacin on the pneumonia model of Pseudomonas aeruginosa (PA) infection in mice, and to explore the method of levofloxacin resistance. Each experiment is based on body weight, randomly divided into groups, using different bacterial concentration and quantity rules to analyze the therapeutic effect of levofloxacin using mouse lung index as an indicator, and to explore its feasibility. Results Low-concentration levofloxacin was effective against a single PA infection (1×109 cfu/mL) mice. The lung indexes of model 1 to model group 2 were significantly different from those of the normal group (Pu003c0.01 Low-concentration levofloxacin was PA (1×10 10cfu/mL) single-infected mice, there are significant differences compared with the normal group, model group 1 and model group 2. (Pu003c0.01). Levofloxacin 3 group and model 2 group comparison. Is there a significant difference in the results ？Levofloxacin 1 and 2 groups can significantly reduce the lung index of the model group. Is there a significant difference between the normal group and the model 1 and 2 groups (Pu003c 0.01)?)? There was no significant difference between levofloxacin 2 group and model 2 group. In each experiment, the weight of the mice on the second day after infection was lower than that of the normal group. There was no significant difference in the results?

　　Conclusion: Under the experimental conditions, low-concentration levofloxacin single-infected PA mice (1 x 1010 cfu/mL)? Low-concentration levofloxacin PA was repeatedly infected in mice, which made the animal model simulate clinical drug resistance to a certain extent.