动物造模,胰岛素,动脉粥样硬化模型 z-bio 2021-06-01 326

　　Objective: To explore the phenotypic differences and pathological mechanisms of insulin-resistant atherosclerosis (IR-AS) models in black-eyed rabbits (WHBE) and Japanese big-eared white rabbits (JW)?

　　Method: WHBE rabbits and 12 JW rabbits were divided into normal control group (NC) and high-fat and high-sugar diet (HF) groups, each with 6? The IR-AS model was induced on the HF diet for 12 weeks and modeled. After completion, blood was collected to measure blood lipid-superoxide dismutase (SOD) and malondialdehyde (MDA) levels; glucose resistance test was performed to calculate the area under the blood glucose and insulin curves; liver microsomal triglyceride transfer protein (MTTP) was detected ); The expression of nuclear factor E2 (Nrf2) SOD1 HE staining to observe the genetic and pathological changes of fat and aortic vessels, and to observe the expression of vascular CD68?

　　Results: Compared with the NC group, the HF group had obesity, elevated blood lipids, glucose intolerance, hyperinsulinemia, and insulin resistance index (HOMA-IR) significantly increased, plasma and liver SOD activity decreased, and MDA content increased. Increased, liver MTTP and Nrf2 gene expression increased, SOD1 gene expression decreased, vascular lipid deposition increased significantly, AS and vascular CD68 expression increased. Compared with the JWHF group, TG, LDL-C, HOMA-IR, area under the glucose tolerance curve (U_GLU), MDA content, fat diameter, liver SOD1 gene expression, AS lesion degree, vascular CD68 expression

　　Conclusion: High-fat and high-sugar diet induces the formation of IR-AS in rabbits, indicating impaired lipid metabolism, inflammation and AS pathology? However, the degree of disease in WHBE rabbits is significantly more severe than that in JW rabbits. Is there a difference between lipid metabolism? And the oxidative stress in the rabbit strain may also be related to the two?