动物造模,流感病毒H3N2 z-bio 2021-12-01 606

　　Purpose: Use the mouse adapted strain of seasonal influenza virus H3N2 to establish a mouse model and explain the pathogenic molecular mechanism of the adapted strain?

　　Method: A/Aichi/2/68 (H3N2) (WT) is more abundant in mice. After the second indication, the mouse indication strain (MA-7) was obtained. MA-7 was nasally infected with BALB/c mice Establish a mouse model with important indicators such as clinical symptoms, weight loss, virus replication, and histopathology, and analyze the adapted strains.

　　Result: The mice were obviously asymptomatic after being infected with WT, and no virus replication was detected. All mice died within 9 days after MA-7 infection, and the weight loss rate exceeded 30? Virus replication and lungs were detectable. The tissue titer is as high as 105.5 TCID50, is there interstitial pneumonia? Gene comparison shows that MA-7 has 5 mutations in HA, NA, PA, NP genes?

　　Conclusion: We have successfully established the H3N2 mouse model-which can be used to study the etiology of the H3N2 influenza virus and its suitable strains for drugs, vaccines, antibody evaluation, etc.