动物造模,裸鼠髓系白血病模型 z-bio 2023-01-30 1132

　　Objective: To establish an M1 leukemia model with BALB/c nude mice?

　　Method: 12 female BALB/c nude mice aged 5-6 weeks were randomly selected and divided into low, medium and high dose groups and blank groups. In the logarithmic growth phase, 3 groups were infused with 3 mice in each group, and the tail vein injections were low, medium, and high, respectively, 1×106, 5×106, and 8×106, and the general condition of the mice was observed. Observed. Peripheral blood was collected at 0, 10, 20, and 30 days after modeling to test blood routine. Perform white blood cell classification. At the 30th day or at the end of the day, the samples were sacrificed and the percentage of CD33CD117 was measured. The positive cells in the peripheral blood and bone marrow were detected by flow cytometry to prepare the tissue. Pathological section?

　　Result: The mouse model has symptoms such as slow motion, reduced motion, and spine arch. 10-15 days after inoculation, on the 30th day of the experiment, the peripheral blood white blood cell count of the high-dose group was significantly higher than that of the blank group (Pu003c0.05); the cell number was significantly higher than that of the blank group (Pu003c0.05); mice in each group The ratio of CD33+CD117+ in the bone marrow increased, and it was the most significant in the high-dose group (Pu003c0.05) u003c0. 05)); there was a small amount of leukemia cell infiltration in the spleen in the high-dose group?

　　Conclusion: BALB/cNude mice were inoculated from the tail vein with 8×106 mouse leukemia cells M1 to construct an acute myeloid leukemia model that is consistent with the biological characteristics of acute myeloid leukemia?