动物造模,经肠道播散诱导内源性感染小鼠模型 z-bio 2021-06-01 306

　　Objective: To establish a mouse model of intestinal disseminated endogenous infection, and to provide a reliable experimental model for studying the related mechanisms of intestinal flora and endogenous infection?

　　Methods: 24 Random model group A, model group B and control group C, model group A Random ICR female mice oral broad-spectrum antibiotic solution destroys the normal intestinal flora, whether 5-fluorouracil (5-FU) is injected from the tail vein Perform immunosuppression? Candida albicans oral introduction of conditional pathogens is based on the model group B. Control group C was treated with saline in the same way? During the experiment, the changes in the fecal flora of the mice were continuously observed. The mouse counts, tissue load, lungs of the mice were detected with the plate, the pathological changes of the liver, cecum and colon tissues were observed by HE staining, and the main intestines of the mice were observed by fluorescence quantitative PCR. Changes in the number of flora?

　　Results: At the end of the experiment, all mice in model A mouse tissues and organs had bacterial infections, and model B mice had mixed bacterial and fungal infections? The lung and liver tissues and organs in the two model groups typically showed inflammation, but the cecum And the colon shows inflammation of the mucosa, and the integrity of the barrier is destroyed. The results of quantitative intestinal flora showed that the structure of the main intestinal flora of the two model groups was disturbed, the intestinal flora was reduced, and the B/E value was less than 1.

　　Conclusion: In mice with intestinal flora disorder and immunosuppression, the intestine or opportunistic pathogens break through the intestinal mucosal barrier, causing tissue and organ infections. Can this model provide a reliable model basis for the prevention and control of endogenous infections from the ecological aspects of intestinal flora?