动物模型,右心房肥大 z-bo 2020-08-06 523

　　(1) Reproduction method Experimental dogs weighing 20-30 kg were anesthetized by intravenous injection of sodium pentobarbital at a dose of 30 mg/kg body weight, and the animals were fixed in a supine position on the right side and were intubated with positive pressure ventilation. Separate the canine forelimb vein and intubate it to the heart to supplement anesthetics; separate the saphenous vein and intubate it to the heart to give experimental drugs. Limb leads, continuous recording of lead Ⅱ ECG. The right femoral artery was separated and intubated, and a pressure transducer was connected to measure the peripheral blood pressure. Cut the skin, open the chest in the third and fourth intercostal spaces, and expose the heart with a stretcher. Cut the open heart capsule and suture it to the chest wall. A Millar micro-tip catheter (Gould PC 350) was inserted along the atrial wall space to measure left atrial pressure (LAP) and right atrial pressure (RAP). Expose and separate the left circumflex coronary artery (LCX), and place an electromagnetic flowmeter probe at the root of the left circumflex coronary artery to measure coronary blood flow. After quickly clamping the vena cava and the odd vein (clamping time does not exceed 3 minutes), cut open the right atrium wall, pass a 0-gauge nylon thread into the right atrium and lead it to the tricuspid valve for a round to form a retractable Ferrule. The right atrium was perfused with Ringer's solution, the right atrium incision was closed with a Satinsky clip, and the right atrium incision was sutured. Release the clamped vena cava and atypical veins, and after the hemodynamic indicators return to normal levels spontaneously, tighten the coil on the tricuspid valve until the right atrial blood pressure rises 1.5 times the left atrial blood pressure (original right atrial pressure: left Room pressure = 0.7). Close the chest cavity layer by layer and suture the wound. 60 days after the operation, the animals were anesthetized again, and various hemodynamic indexes were measured. Finally, the animals were sacrificed, the heart was taken to measure the tricuspid valve aperture, the vena cava was taken to measure the diameter, the liver and spleen were weighed and embedded in sections for pathological examination.

　　(2) Features of the model The heart valve shrinkage is confirmed, but it requires high experimental technology. The operation must be fast and must be completed within 3 minutes. However, due to the greater damage caused by thoracotomy, the animal is more likely to die.

　　(3) Comparative medicine Tricuspid valve stenosis is the most common clinical cause of rheumatic coronary heart disease, which is rare alone, and is often accompanied by insufficiency, mitral valve and aortic valve damage. This model only achieves tricuspid valve stenosis by tightening the gap of the tricuspid valve, which cannot reflect the various pathological characteristics of clinical tricuspid valve stenosis.