动物造模,肠道过敏经皮致敏模型 z-bio 2021-06-02 724

　　Objective: How to establish a transdermal sensitization model of intestinal allergy in mice?

　　Method: Firstly, compare the degree of intestinal allergies between percutaneous sensitization (skin on the back of the ear) and intraperitoneal injection sensitization. Choose a sensitive and easy operation method. The skin sensitization method of the ear is to apply calcipotriol ointment to the mouse. Cause skin irritation on the ears, then apply obavmin (OVA). Have you sensitized mice for 2 weeks? IgE (totalIgE, tIgE) and mouse obesity cell protease-1 (mousemastcellprotease-1, mMCP-1) levels and other indicators for evaluating pathological changes and air-intestinal tissue models?

　　Results: Compared with the transdermal sensitization intestinal allergy model, can the transdermal sensitization intestinal allergy model induce higher plasma tIgE and mMCP-1 levels in the peritoneal sensitization model? The transdermal sensitization model can induce higher levels of plasma tIgE and mMCP-1? Higher plasma tIgE and mMCP-1 levels. After co-administration of OVA, mice in the model group began to develop symptoms of intestinal allergies such as rapid scratching, bending over, diarrhea, rectal temperature decreased (about 1.2°C), and jejunal capillary permeability increased (about 1.2°C) twice . ), plasma tIgE and mMCP-1 are significantly increased, and jejunal mucosa is damaged by inflammatory cell infiltration? Increased mast cells (about 5x)?

　　Conclusion: The mouse model of percutaneous sensitizing enteritis has typical pathological features of food and intestinal allergies. It represents the sensitization pathway of skin exposure to allergies and provides new research tools for its mechanism. Food allergy research and preventive measures?