动物造模,鼠夹层动脉瘤动物模型 z-bio 2021-06-02 388

　　Objective: To study the effect of β-aminopropionitrile (BAPN) on the aortic wall of mice, and to further explore an aneurysm anatomical animal model that meets the characteristics of human diseases and the characteristics of human aneurysms.

　　Methods: BAPN and laboratory mixed animal drinking water prepared with a concentration of 0.2? 0.4? 0.6 4-5 weeks old SPF grade SD rats and 3 weeks old SPF grade C57BL/6 mice BAPN solution, raised for 7 weeks, died or anatomy , Separate the aorta and observe the changes of the whole body. The aorta is divided into four parts: ascending aorta, descending aorta, abdominal aorta, suprarenal artery, and abdomen. The segments of the aorta, inferior renal artery and blood vessel are intercepted and the surface is stained with HE , Measure various indicators such as the inner diameter of the blood vessel and the area of the media. At the same time, the type A dissected aneurysm aorta from open thoracic surgery was collected for HE staining to observe the pathological changes and anatomical development. Comparison of rat aorta?

　　Results: 1) Does BAPN have a significant effect on water intake and intake? Rats or mice gain weight? 2) Does BAPN expand the aorta, thicken the rat or mouse mesentery and reduce elastin? ? Sequence disorder, its pathological changes conform to the anatomical characteristics of human aneurysms? 3) The concentration 0.4 BAPN solution model has the highest success rate?

　　Conclusion: C57BL/6 mouse aneurysm anatomical animal model can be used as a simple, economical and effective model. The next animal model: The incidence of systemic pathological changes such as intestinal rupture in SD rats is higher than the incidence of anatomical aneurysms? The incidence of lateral lumbar scoliosis is higher than the incidence of anatomical aneurysms. Is further research necessary as an animal model for anatomical aneurysms?