动物造模,人柯萨奇B2病毒感染恒河猴模型 z-bio 2021-06-02 523

　　Objective: To conduct a preliminary analysis of the oral infection characteristics of rhesus monkeys infected with human Coxsackie B2 virus, and establish experimental methods and technical support for the establishment and subsequent development of human Coxsackie B2 virus rhesus monkey infection models. Basis of research on infection mechanism and pathophysiology.

　　Method: 3-4 months old red-throated monkey cubs were orally infected with Coxsackie B2 virus, the clinical symptoms and body temperature of Renesas monkeys were observed and recorded, and blood was drawn for physiological and biochemical indicators. Blood samples and herpes virus detection; herpes histopathological examination.

　　Results: Red lizards developed hand, foot and mouth herpes 2-5 days after infection; animals showed depression, reduced activity and diet; body temperature showed no fever; Ke Saatchi B2 virus samples were detected in both herpes fluid and blood; normal blood tests It showed that white blood cells decreased, monocytes increased, lymphocyte count decreased, granulocyte count increased, and eosinophils increased, which is conducive to the decrease of basophils. Blood biochemical examination showed that liver function, kidney function, and myocardial enzymes all increased to varying degrees. Herpes Herpes is manifested by thickened flat epithelium, superficial hyperkeratosis, local necrosis with inflammatory cell infiltration and abscesses.

　　Conclusion: After the human Coxsackie B2 virus infects the young monkeys, a series of more characteristic hand-foot-mouth disease, clinical symptoms, physiological and biochemical symptoms, viremia and other symptoms appear in the red lizard monkeys. It shows that the infection method can be successfully constructed. For the animal model of Ko Saatchi B2 virus infection, it is feasible to establish and related detection methods and technologies. The next step is to establish the red-footed monkey Kokusakki B2 virus infection model, pathogenic research, and experimental basis for the evaluation of Lay drugs and viruses.