动物造模,大鼠高尿酸血症模型 z-bio 2021-06-02 684

　　Objective: To establish a rat model of hyperuricemia to explore whether hyperuricemia can cause secondary cardiovascular disease.

　　Methods: Randomly select 32 SPF male SD rats from the normal control group (C group), potassium oxalate model group (M1 group), and oxazine potassium model combined with high-sugar and high-fat diet group (M2 group), and extract them from yeast The animal feed model group (M3 group) was divided into the oxazine potassium acid group, with 8 animals in each group, and the model was made for 3 consecutive weeks. The pathological test results are supplemented by comparing rat serum uric acid (UA), urea nitrogen (BUN), creatinine (Cr), insulin (INS), blood sugar (GLU), triglyceride (TG) and other indicators. , To study the rat model of hyperuricemia.

　　Results: The UA level of hyperuricemia model rats replicated by forced oral administration of 750 mg/kg of potassium oxalate combined with yeast extract feed was significantly increased (pathological changes in P rats), which caused hyperuricemia, but it was also accompanied by 3 /8 Rat heart GLU, INS, TG and pathological changes, suggesting the development of secondary cardiovascular disease.

　　Conclusion: The modeling method of potassium oxalate combined with yeast extract feed is more suitable for establishing a long-term rat hyperuricemia model than the potassium oxalate model alone. In addition, secondary blood glucose disorders, therefore, the model construction program can be used as an animal model to study the mechanism of the interaction between hyperuricemia and cardiovascular disease, and can also be used for preclinical pharmacokinetic studies.