动物造模,2型糖尿病小鼠模型 z-bio 2021-10-09 699

　　Objective: To observe the changes of blood glucose in a mouse model of type 2 diabetes, and to explore the feasibility of using blood glucose 2 hours postprandial as an evaluation point for blood glucose intervention.

　　Method: Choose healthy C57BL/6 mice, use high-fat diet and streptozotocin (STZ) to guide the type 2 diabetes (T2DM) experimental group and set up a normal control. Measure the continuous fasting blood glucose and continuous random blood glucose respectively, analyze the blood glucose change rule of the mice, and evaluate the scientificity of the blood glucose 2 hours postprandial as the evaluation point of blood glucose intervention in T2DM mice. Long-term observation of blood glucose fluctuations after meals in diabetic mice for 2 hours, to verify the stability of the blood glucose intervention evaluation points for 2 hours after meals.

　　Results: The continuous fasting blood glucose level of T2DM mice showed a relatively stable range of blood glucose level changes during the 2-3 hour fasting period after a meal. After fasting for 4 hours or more, the blood glucose level of the T2DM group mice continued to decrease and change. The blood glucose level was significantly reduced, and the normal group was able to maintain a slow rise, and the blood glucose level was relatively stable; within 7 weeks, the blood glucose fluctuation of 2 hours after meal in T2DM mice was the blood glucose of 2 hours after meal.

　　Conclusion: The blood glucose level 2 hours after a meal can stably reflect the blood glucose change rule of the T2DM mouse model, and it has excellent application value in the evaluation of blood glucose intervention experiments.