动物造模,酒精性心肌病小鼠模型 z-bio 2022-11-14 1038

　　Objective: To establish a mouse alcoholic cardiomyopathy model through a liquid diet, and to provide an excellent animal model for studying the pathogenesis of alcoholic cardiomyopathy.

　　Method: 16-week-old C57 mice (n=21 in the alcohol group, n=9 in the non-alcohol group) were fed with alcoholic liquid feed and non-alcohol control feed. The alcohol concentration was gradually increased from 4.8? to 5.4 with the alcoholic feed. ? High, fed for 8 weeks, weighed weekly, monitored mortality, and performed echocardiography and pathological examinations after 8 weeks.

　　Result: The drinking group began to lose weight from the 2nd week and continued until the 8th week. The weight was significantly reduced compared with the control group. At the same time, seven mice in the alcohol group died. The control group had no deaths during the drinking period (33.3 occupancy); echocardiography results showed that the anterior and posterior walls of the ventricle in the alcohol group were significantly thinner than the control group, the ventricular diameter increased, and the cardiac ejection fraction and short axis shortening rate were significantly reduced; alcohol The group is smaller. The mouse heart/body mass index increased significantly compared with the control group. But the HE staining results also showed a significant increase in the heart. Mice in the alcohol group.

　　Conclusion: After 8 weeks of alcohol infusion, the mouse heart showed typical pathological features of alcoholic cardiomyopathy, including overall hypertrophy, thinning of the left ventricular wall, and decreased heart function. Therefore, this method can successfully prepare a mouse model of alcoholic cardiomyopathy.