动物造模,颅脑损伤后认知障碍大鼠模型 z-bio 2021-12-27 758

　　Objective: To discuss the establishment and evaluation of animal models of cognitive impairment after craniocerebral trauma.

　　Method: 60 healthy male rats (Sprague Dawley, SD) and 48 water mazes were screened experimentally, and they were randomly divided into blank control group, model control group and model group. An electronic brain injury device (electroticalContusionImpactor, eCCI) was used to prepare a model, and combined with water maze screening to identify rats with cognitive impairment after traumatic brain injury (TBI). Observation of vital signs, nerve function and motor function of rats, Morris water maze test, determination of serum MMP-2, MMP-9, SOD, MDA content changes.

　　Results: Compared with the blank group: the vital signs of the model group were stable, the behavior indicators were different (Pu003c0.05), and the results of the water maze experiment latency were significantly different. The space exploration experiment (Pu003c0.01) serum MMP-2, MMP-9 and MDA levels Significantly increase (Pu003c0.01) SOD significantly decrease (Pu003c0.01)..

　　Conclusion: This modeling strategy can better simulate the cognitive impairment after craniocerebral injury, and at the same time has the characteristics of stability, repeatability, objective evaluation, and convenient operation.