动物造模,阿兹海默病,β淀粉样蛋白 z-bio 2021-06-07 771

　　Inoculating mice with brain homogenate from patients with sporadic Alzheimer's disease can cause brain amyloid to deposit on the receptor, which suggests that β-amyloid (Aβ) peptides can self-proliferate as prions.

　　Stanley Prussiner et al. tested whether the brain was inoculated with plaques caused by two synthetic β-amyloid (Aβ) peptides (consisting of 40 or 42 amino acids and polymerized into amyloid fibrils). This is the Alzheimer’s Features of silent disease. The authors report that this synthetic β-amyloid (Aβ) peptide 40 preparation composed of elongated fibers completely produced both peptides in the corpus callosum, hippocampus, and cerebral cortex of recipient mice. I'm. 42 is composed of short fibers, with fewer and fewer plaques, and mainly contains β-amyloid (Aβ) peptides. 42 However, treatment of these amyloid preparations with a low concentration of detergent SDS before inoculation can eliminate the observed plaques Differences in block content, abundance and distribution. The author believes that the types of amyloid β (Aβ) that may cause pathological heterogeneity in patients with Alzheimer's disease may help to grade the treatment of patients. I'm. in

　　In a related study, Prusina and his colleagues tested whether different amyloid β (Aβ) aggregates are distributed in the brains of different Alzheimer's disease patients. When the authors used brain homogenates from two patients with different inherited Alzheimer's disease to inoculate transgenic mice, these patients developed an Arctic mutation that changed the sequence of these β-amyloid (Aβ) peptides. It either has a Swedish mutation sequence that appears outside of these. Various patterns and abundant amyloid deposits appear in the brain. In addition, as these β-amyloid (Aβ) types continue to grow in mice, these differences persist, indicating that these types have prion-like transmission capabilities. The authors found that monoclonal antibodies developed for passive Alzheimer's disease immunotherapy against certain types of amyloid (Aβ) may not be effective against other types of amyloid (Aβ). He said he had sex and had shown a series of failures of this method in the past. This is rational.