动物造模,巨噬细胞 z-bio 2021-06-07 829

　　Purpose requirements

　　1. Understand the principle of phagocytosis of mouse peritoneal macrophages

　　2. Familiar with the basic process of phagocytosis

　　Fundamental

　　The phagocytosis of

　　 cells is a way of ingesting nutrients in unicellular animals. Macrophages, monocytes and neutrophils in higher animals have phagocytic function and are an important part of the body's non-specific immune function. Macrophages are generated by differentiation of bone marrow stem cells. When pathogenic microorganisms or other foreign bodies invade the body, macrophages will accumulate at the foreign body due to their chemotaxis. The macrophages can internalize them into the cytoplasm to form phagocytic vesicles. , And then fuse with lysosomes intracellularly to digest and decompose foreign matter. The formation of phagocytic vesicles requires the help of microfilaments and their binding proteins. If the cells are treated with the drug cytochalasin B that degrades microfilaments, the formation of phagocytic vesicles can be blocked; the immunosuppressant cyclophosphamide (CYP) can affect macrophages. The phagocytosis of cells also has an impact.

　　Experimental supplies

　　1. Equipment: microscope, dissecting dish, scissors, tweezers, syringe, glass slide, cover glass, pipette, etc.

　　2. Reagents:

　　0.85% normal saline; Alsever blood preservation solution; 6.0g of soluble starch, add 100ml of water and boil for later use.

　　Three days before the experiment, 1ml of soluble starch was injected into the abdominal cavity of mice every day, and 1ml of 1% chicken red blood cells were injected into the abdominal cavity 30 minutes before the experiment.

　　3. Material: mice; 1% chicken red blood cell suspension

　　Methods and steps

　　After the blood is taken from the inferior wing vein of the chicken, it is centrifuged twice with normal saline, and then an appropriate amount of normal saline is added according to the volume of the red blood cells obtained to adjust the concentration of the chicken red blood cells to 1%.