动物造模,重度创伤失血性休克模型 z-bio 2021-06-08 560

　　Objective: To establish an improved rat model of severe traumatic hemorrhagic shock, and to explore the protective effect of anesthesia on the establishment of STHS rat model.

　　Methods Forty healthy male SD rats were selected and randomly divided into 4 groups. The normal group was awakened by bilateral femoral artery and left femoral vein cannulation anesthesia, waited and maintained anesthesia, and divided into awake control group (CC, = 10) , Anesthesia control group (AC, n = 10), traumatic shock group was anesthesia combined with open wound or anesthesia. Routine quantitative control of blood loss 50? Use 50? rat model traumatic hemorrhagic shock group (CTHS, = 10), anesthesia traumatic bleeding, respectively cause severe traumatic hemorrhagic shock. Sexual shock group (ATHS, = 10). Observe the general physiological state of each group of rats during the experiment, and continuously monitor the hemodynamic indicators of the experimental rats: mean arterial pressure (MAP), heart rate (HR), maximum left ventricular pressure increase and decrease rate (±dp) /dtmax )), arterial blood gas index: pH, oxygen partial pressure (PaO2), carbon dioxide partial pressure (PaCO2), hemoglobin measurement (ctHb), lactic acid (Lac), blood sugar (Glu), bicarbonate concentration (cHCO3-), heavy carbon Salt residue (BE); at the end of the experiment, observe the damage of the rat's heart, liver, lung, and kidney organs under a HE staining microscope.

　　Results There was a significant difference in the time course of HR and ±dp/dtmax between the CTHS group and the ATHS group (Pu003c0.01). The HR and ±dp/dtmax of the ATHS group showed a downward trend, but the CTHS group showed a downward trend, first decreasing (T1) and then increasing High, elevated or stable (T2, T3, T4, T5, T6) state; MAP showed a gradual upward trend after electric shock in ATHS group, and finally returned to normal level at 25-0, while CTHS group declined rapidly after compensating for the growth period. PH, PaCO2, ctHb, cHCO3-, BE showed a gradual decrease trend in CTHS group and ATHS group. Except for PH and ctHb, the decline in CTHS group was significantly greater than that in ATHS group (Pu003c0.01).); Glu, Lac, PaO2 It showed a gradual upward trend, and the increase in CTHS group was significantly higher than that in ATHS group (Pu003c0.01). Compared with the CTHS group, the liver, heart, lung, and kidney damages in the ATHS group were significantly reduced.

　　Conclusion Anesthesia has a protective effect on the establishment of severe traumatic hemorrhagic shock in rats, which can improve hemodynamics, reduce respiration and metabolite accumulation, and reduce the degree of damage to various organs.