动物模型,急性胃溃疡 z-bo 2020-08-07 775

　　(1) Copy method Adult rats, fasting and water for 48 hours, anesthetize with ether, incise the abdominal wall, expose the stomach, draw the stomach out of the abdominal cavity with blunt forceps, and ligate it with surgical suture at the junction of the pylorus and duodenum , And then put the stomach back in situ and suture the abdominal wall. After the operation, the animals were placed in wire cages, fasted and water-free, 15 to 19 hours later, bloodletting was sacrificed, the whole stomach was removed, the gastric juice was collected, 3000r/min, centrifuged for 10 minutes, the supernatant was sucked, its volume was measured, and its acidity and acidity Pepsin activity, then flatten the stomach along the greater curvature of the stomach, observe the mucosal ulcer damage, calculate the number of ulcers and ulcer area, convert them into ulcer index, and perform histomorphological examination.

　　(2) Model characteristics Model animals have stagnant gastric juice, increased total acidity, increased pepsin activity, and anterior gastric mucosal congestion and edema, with obvious bleeding points and punctate, strip-shaped ulcers or necrotic foci. Under the light microscope, leukocyte infiltration and tissue necrosis were seen, and the ulcer reached deep into the muscle layer.

　　(3) Comparative medicine The pyloric ligation method is Shay's traditional classic rat gastric ulcer model method. After ligation of the pylorus, gastric juice is retained in the stomach, and the defense ability of the gastric wall is weakened, leading to the formation of ulcers. This model usually uses the total acidity of gastric juice, pepsin activity, ulcer index and other indicators to determine the condition of the disease, and the model replication success rate is 85% to 100%. It is worth mentioning that the degree of gastric ulcer damage caused by this model is significantly related to various factors during operation. When copying, special attention should be paid to the fasting of the animal and the time elapsed after ligation to control the observation index Maintain good stability. Among animal models of acute gastric ulcer, this model is also one of the most classic and commonly used methods.