动物造模,过敏性结膜炎小鼠模型 z-bio 2021-10-14 400

　　Objective: To establish a mouse model of allergic conjunctivitis with different antigens, different excitatory pathways, and different ages, compare the immune response status, use it as the best mouse model of allergic conjunctivitis, and screen the crop status. Establish and provide new ideas and methods.

　　Methods: 65SPFBalb/c mice, different antigens (ovalbumin, pigeon pollen, mice), different excitatory routes (forced feeding, spraying, subcutaneous injection), different mouse ages (1 week, 2 weeks, 3 weeks old) 8 weeks old) Establish a mouse model of allergic conjunctivitis). T-qPCR was used to detect the relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctival tissues, and flow cytometry was used to detect IL CD4+T cells in splenic single cell suspension. -17? And detect

　　Result: Compared with the negative control group, the expression rates of IL-17, CCL5 mRNA and IL-17 mRNA in the ovalbumin group, house dust mite group and ragweed pollen group all increased. CCL5 mRNA and IL-17 mRNA increased in IL-17 and ragweed pollen groups. the most important. To increase. Compared with the negative control group, the expression ratio of IL-17 and IL-17 mRNA in the subcutaneous injection group, gastric administration group, nebulized inhalation group and positive control group increased, and the expression of CCL5 mRNA in the gastric administration group was similar... ...It used to be. Negative control group. The expression level of this group was close to the positive control group. The expression levels of IL-17, CCL5 mRNA and IL-17 mRNA were similar in 1-week-old and 2-week-old mice, but were significantly lower than 3-week-old and 8-week-old mice. ..

　　Conclusion: Ragweed pollen has excellent allergenicity in establishing mouse models of allergic conjunctivitis. Subcutaneous injection of stimulating antigen can achieve satisfactory results in the structure of mouse allergic conjunctivitis animal model. There are no obvious eye symptoms or signs, but the body and parts are sensitive. The immune response of 3-week-old Balb/c mice is similar to that of adult Balb/c mice.