动物造模,诱导小鼠慢传输型便秘模型 z-bio 2021-10-11 402

　　OBJECTIVE: To compare mouse models of delayed constipation induced by loperamide hydrochloride, compare the differences in the models induced by different doses and different administration times, and establish a more stable mouse model of delayed constipation induced by loperamide hydrochloride .

　　"Method: Use healthy male C57BL/6 mice to prepare 0.025, 0.25 and 2.5 mg/mL loperamide hydrochloride suspensions by gastric administration. The administration time is twice a day at 9 am and 3 pm. The effect of the model was evaluated from the detection of intestinal propulsion rate and stool volume, and the effects of different doses and different administration times on the slow transit constipation mouse model were observed.

　　Results: 1) Compare the effects of different doses of loperamide hydrochloride in the concentration range of 0.5mg/kg～50mg/kg on model building. By comparing the intestinal propulsion rate between the groups, the result is 50mg, which indicates that the control group is 50mg/kg In comparison, the intestinal advancement rate and stool volume of the blank group mice were significantly reduced, and the difference was statistically significant (P\u003c0.05).

　　Conclusion: The study found that continuous intragastric gavage at a dose of 50 mg/kg for 7 days can significantly reduce the rate of intestinal progression in mice and significantly reduce the amount of defecation. This allows you to create a relatively stable mouse model of impaired colonic transport. Constipation: The study of the slow transit constipation model caused by loperamide hydrochloride provides valuable data.