动物造模,抑郁症模型 z-bio 2021-09-29 422

　　Objective: To establish an animal model of long-term stress depression.

　　Method: 17C57BL/6J 4-week-old mice weighing (11.05±1.65) g were randomly divided into control group and long-term psychological stress stimulation (LTMS) group. The LTMS group was kept in a cage, fed the cats for 21 days, shocked for 3 hours a day, and deprived of sleep for 12 hours. The experimental group measured changes in food intake, elevated cross-maze experiments, field experiments, and changes in forced swimming behavior to determine whether the modeling was successful.

　　Results: Compared with the control group, the food intake of the LTMS group was significantly reduced (Pu003c0.01), and the time of forced swimming immobility was significantly prolonged (Pu003c0.01). In the open field test, the number of crossing grids and the number of posts in the LTMS group were significantly lower than those in the control group, and the difference was very significant (Pu003c0.01). In the elevated cross maze experiment, the residence time of the closed arms of the mice in the LTMS group was significantly prolonged, and the difference was very significant (Pu003c0.01). The percentage of time to enter the open arm decreased, and the difference was significant (Pu003c0.05).

　　Conclusion: The symptoms shown by the long-term psychological stress model are similar to clinical symptoms such as behavioral despair, inconvenience, loss of interest, anxiety and depression, and can be used as a depression model.