动物造模,压力超负荷心衰小鼠模型 z-bio 2021-06-16 733

　　Objective: To establish a mouse heart failure model, using ultrasound imaging and pathology techniques to comprehensively evaluate the characteristic changes in cardiac function and structure during the onset of disease.

　　Method: Aortic arch stenosis (lateral aortic stenosis, TAC) surgical technique was used to establish a pressure-overload heart failure model in mice. Doppler was collected at 0 weeks and 2, 4, and aortic arch stenosis before blood flow. 8 weeks after modeling. Estimate vascular pressure and collect B-mode and M-mode ultrasound images to evaluate the left ventricular systolic function of the heart structure, and combine the mitral valve annulus to collect the mitral valve orifice blood flow Doppler.. Used to evaluate the expansion function and collect the heart tissue Tissue Doppler. Histopathological observation.

　　Results: Ultrasound diagnosis showed a significant increase in aortic blood pressure at 2, 4, and 8 weeks after the operation. Two weeks after the operation, the main symptoms were a characteristic compensatory increase in left ventricular wall thickness, a decrease in inner diameter, and an increase in compensatory systolic function, but accompanied by impaired diastolic function and myocardial fibrosis. Four weeks after the operation is a transitional period. The main manifestations are the transition from ventricular wall hypertrophy to ventricular dilatation, systolic function begins to decline, diastolic function continues to be impaired, and the degree of myocardial fibrosis increases; 8 weeks after surgery, the characteristic ventricular cavity dilates, and myocardial cells show both systolic and diastolic functions. Dissolved and fibrosis was evident.

　　Conclusion: The structure and function of the left ventricle are evaluated in three stages: compensatory myocardial hypertrophy, aortic arch contraction in mice to decompensated dilated heart failure, aortic arch contraction model basis, and translational research.