动物造模,复杂性区域疼痛综合征 z-bio 2021-06-16 483

　　Objective: To observe the therapeutic effect of electroacupuncture at "Zusanli" and "Kunlun" points on mechanical hyperalgesia in a rat model of complex local pain syndrome type I, and observe the therapeutic effect of electroacupuncture on CRPS-I rat model electroacupuncture. Bottom tissue The intervention effect of oxidative stress is to study the analgesic mechanism of treatment.

　　Method: Use an O-shaped rubber ring to compress the ankle joint, and establish a chronic post-ischemic pain model (CPIP) to simulate CRPS-I. Healthy male SD rats were randomly sham group, CPIP model group (CPIP group), CPIP model + electroacupuncture group (CPIP+EA group) and CPIP model + sham electroacupuncture group (CPIP+). Electroacupuncture was performed every day from 1 day after the model. After 30 minutes of electroacupuncture intervention, the 50-degree withdrawal threshold (PWT) of the affected side of the rat was measured by the von Frey method. A molecular detection kit was used to detect the affected side of the rat. Expression of oxidative stress-related molecules in plantar tissues. Immunofluorescence method was used to detect the expression of cell oxidative damage marker 8-OHG in rat plantar tissues.

　　Results: Compared with the Sham group, the CPIP group had a significantly lower 50?T of the plantar (Pu003c0.01), and it continued until the experimental observation was completed and the model was normal. Description has been established in. The 50?T of the affected side in the CPIP+EA group was significantly higher than that in the CPIP+EA group (Pu003c0.01); the superoxide dismutase (SOD) was compared with the sham operation group. ) And gluten reduction increased significantly on the affected side of the CPIP+EA group (Pu003c0.01). The level of cystathionine (GSH) was significantly reduced, and the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and the expression of 8-OHG were significantly increased (Pu003c0.05 or Pu003c0.01); significant after electroacupuncture Increase hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels and 8-OHG expression (Pu003c0.05 or Pu003c0.01). Compared with the CPIP+ShamEA group, the SOD and GSH levels of the plantar tissues of the CPIP+EA group were significantly increased, and the H2O2, MDA content and 8-OHG expression were significantly decreased (Pu003c0.05 or Pu003c0.01).

　　Conclusion: Electroacupuncture can significantly reduce the level of oxidative stress in the plantar tissue of CPIP model rats.