动物造模,大鼠肝外型胆汁淤积模型 z-bio 2021-06-16 811

　　Objective: To evaluate the advantages and disadvantages of three surgical models of extrahepatic cholestasis in rats through serology, imaging and pathology.

　　Methods: Fifteen female SD rats were randomly divided into a conventional group, a modified group, and an electrosurgical group. The common bile duct ligation (n=5), the common hepatic duct suture (n=5)) and the common bile duct were... Common duct radiofrequency electrosurgery electrocoagulation (n = 5) was used to simulate cholestasis surgery. Before and after the operation, CT scan and weight measurement were performed to compare liver density and bile duct diameter. .. Rats in group 3 were bled 7 days after the operation. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), common bile duct (TBIL), direct bile duct (DBIL); rats were anesthetized and overdose after blood sampling. , Take part of liver tissue, fix it with Ermarin solution 4.2?.2?ess, and observe the pathological changes in paraffin sections with HE staining.

　　Results: The three groups of rats all developed liver damage and cholestasis to varying degrees 7 days after the model was created. The AST, TBIL and DBIL of the traditional group were higher than those of the electrosurgery group (P0.05). Pathological results showed that bile duct hyperplasia and bile duct wall thickening in the conventional group were the most serious among the three groups. CT showed that the dilated diameter of extrahepatic bile duct in the conventional group was larger than that in the modified group and the electrosurgery group (P\u003c0.05). CT value. The proportion of liver in the traditional group was significantly lower than that before surgery (P\u003c0.05).

　　Conclusion: Both electrosurgery and improved methods can more effectively prolong the course of cholestasis in rats, and can better simulate some human extrahepatic cholestasis, providing the possibility for dynamic observation of the pathological process of cholestasis.