动物造模,门脉高压动物模型 z-bio 2021-06-16 893

　　Objective: To investigate whether a simple ligation of the left portal vein can form a stable portal hypertension, and whether nitric oxide plays a corresponding role.

　　Method: 36 SPF male 8-week-old SD rats were randomly divided into left branch ligation group (needle diameters of 0.6, 0.7 and 0.8 mm) and main stem ligation group (diameter of 0.9 mm). There are 6 groups of acupuncture), left external lobectomy group and sham operation group. The portal pressure of all rats was measured before surgery, immediately after surgery, 15 days after surgery, and 30 days after surgery. All rats were sacrificed on the 30th day after surgery, and portal vein blood and left and right liver tissues were collected to determine the content of nitric oxide (NO, NO, NO).

　　Result: Immediately after operation, 15 days and 30 days later, the portal vein pressure of SD rats in the left branch ligation group was significantly higher than that before operation and the control group. The difference between the left branch ligation group and the supervisor was 0.6 mm. There was no statistically significant difference in blood and liver tissue NO content in the left and right liver tissues of the 6 groups of SD rats, and there was no significant difference in the 0.9 mm trunk group at 30 days postoperatively.

　　Conclusion: Simple ligation of the left portal vein can form stable portal hypertension, and a 0.6mm diameter needle can achieve better results.