动物造模,大鼠肥胖模型 z-bio 2021-10-12 834

　　Objective: To establish a rat obesity model using a high-calorie diet, observe the differences between the two rat models, compare different obesity assessment methods and obesity-sensitive animal screening methods, and provide an animal model reference for studying the functionality of the product for weight loss.

　　Method: 120 SD and Wistar male rats were selected and randomly divided into two groups (blank control group and model group) according to their body weight, and 60 rats were placed in each group. The blank control group was fed maintenance feed, and the model group was fed a modified high-calorie feed formula. The weight and length of the rats were recorded once a week, and the feed quality was weighed twice a week. Calculate Li's index, food intake and food utilization. 6 weeks. After feeding for 2 or 6 weeks, 30 rats in each group were selected for blood biochemical index detection. After the dissection, the fat around the kidneys and the fat around the testicles were collected and weighed, and the fat/body ratio was calculated. Analyze the correlation between rat obesity index, Lee index, obesity and obesity susceptibility, exclude the increase in obesity, which ranks in the bottom 1/3, and then compare the increase in obesity and obesity in rats after 2 weeks of feeding. , 10 rats and 20? correlation.

　　Results: Compared with the blank control group, the body weight, Lee index, weight gain, food utilization, body fat mass, fat/body ratio, food intake decreased, and serum glucose levels of the two strains in the model group increased. increased. The level of acylglycerol increased and the level of high-density lipoprotein cholesterol decreased (both P0.05). There were occasional differences in the length, Lee index, and food consumption of rats in the blank control group (all P). u003c0.05). There were significant differences in serum HDL cholesterol levels in blank control rats fed for 2-6 weeks (Pu003c0.05). The correlation between weight gain of two rats and susceptibility to obesity and obesity was significantly greater than the correlation between Lee index and susceptibility to obesity and obesity (both Pu003c0.05). Excluding weight gain, the correlation between weight gain of 10-20? rats and obesity was significantly better than that of excluding the lower 1/3 of rats. Excluding 10? rats, the correlation coefficient was the highest.

　　Conclusion: SD and Wistar rat obesity models can be successfully established through an improved high-calorie diet, and there is no significant difference between the two species. Weight gain is better than Lee's obesity assessment index. Screen obesity-sensitive animals. For obesity-sensitive animals, you can choose to eliminate weight gain by 10?