动物造模,盐敏感性高血压小鼠模型 z-bio 2021-10-12 667

　　Objective: To establish a salt-sensitive hypertensive mouse model, and use the antihypertensive drug nifedipine to verify the model.

　　Method: C57BL/6J mice were studied, and the normal group fed with a regular diet, the high-salt group fed with a high-salt diet, and an intervention group with nifedipine after the high-salt diet were set up. .. Use a non-invasive blood pressure measurement system to measure the blood pressure changes of each group of mice. The blood biochemical analyzer is used to determine biochemical indicators such as liver function, kidney function, blood lipids, blood sugar, and ions. Mouse serum; hematoxylin-eosin staining is used to observe the small histological changes of rat liver, kidney and carotid artery.

　　Results: The systolic and diastolic blood pressures of the mice in the high-salt group were significantly higher than those of the normal group (both Pu003c0.01), while the blood pressure of the intervention group mice was significantly increased and dropped to (all Pu003c0.01) ). Compared with the normal group, the liver function indexes of the mice in the high salt group were aspartate aminotransferase, alkaline phosphatase, albumin, total serum protein and direct bilirubin levels, the blood lipid indexes were cholesterol, and the kidney function indexes were uric acid and calcium. The intervention group gradually recovered, and the ion and magnesium ion levels changed significantly (both Pu003c0.05). Histopathology showed that the liver and kidney of the mice in the high salinity group had varying degrees of damage.

　　Conclusion: High-salt diet can successfully establish a salt-sensitive hypertensive mouse model, and nifedipine can effectively reduce the blood pressure of the model mice.