动物造模,高原肺水肿模型 z-bio 2021-10-12 733

　　Objective: To study the influence of various hypoxic stress methods on the establishment of a high altitude pulmonary edema model.

　　Method: 60SD rats were randomly divided into 3 groups: control group (altitude 400m), hypobaric chamber group (simulated hypoxic stress at 6000m altitude for 48h), field hypoxia group (altitude 4200m hypoxic stress for 28d); 20 groups. The lung tissue of experimental rats was changed by detecting the dry-wet ratio, morphological and pathophysiological characteristics, the expression of the main genes aquaporin-1 (AQP-1) and vascular endothelial growth factor (VEGF), and the level of oxidative stress to compare their effects . Hypoxic stress method to construct SD rat model of high altitude pulmonary edema[J].

　　Results: Compared with the control group, the pulmonary artery pressure and lung tissue water content of rats in the hypobaric oxygen chamber group and the field hypoxia group were significantly increased (both Pu003c0.01), and the oxygen partial pressure and lung tissue water content were significantly increased. High (Pu003c0.01). The oxygen saturation decreased significantly (both Pu003c0.01). The lung tissue of the control group showed normal structure under light microscope and electron microscope. The lung tissue of the hypobaric oxygen chamber group and the field hypoxia group showed alveolar walls under the light microscope, and the alveolar spacing was obviously diffused. The alveolar cavity is filled with a large number of red blood cells and inflammatory cells, and there is obvious edema. The levels of AQP-1 mRNA and protein in lung tissues of the two experimental groups were significantly higher than those of the control group (both Pu003c0.01), and the levels of VEGF mRNA and protein were significantly lower than those of the control group. In the control group (all Pu003c0.01).u003c0.01).all Pu003c0.01), serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were significantly reduced , Malondialdehyde (MDA) levels increased significantly.

　　Conclusion: The hypobaric oxygen chamber simulates the 48-hour high altitude pulmonary edema model of SD rats at an altitude of 6000 meters and 4200 meters of field hypoxia stress for 28 days.