动物造模,化疗损伤性卵巢早衰模型 z-bio 2021-10-08 472

　　Objective: To explore the method of establishing a rat model of primary ovarian insufficiency caused by chemotherapy by intraperitoneal injection of different doses of cisplatin (CP).

　　Method: Take 48 female fetal Wisar rats and divide them into 6 groups, each with 8 rats. Different doses of CP were injected intraperitoneally at different times. The control group (group A) was untreated; CP intraperitoneal low-dose (1.5mg/kg) injection group 1 (group B), low-dose (2.0mg/kg) injection group 2 (group C) and low-dose (2.5mg/kg) ) Injection group 3 (group D), all once a day for 5 consecutive days; high-dose group 1 group E CP intraperitoneal injection), weekly 4 mg/kg, 4 mg/kg injection; CP intraperitoneal injection high-dose group 2 F) , 4 weekly injections of mg/kg and 6 mg/kg to establish a POF model induced by chemotherapy in rats. Observe the general condition of the rats, the estrus cycle, and detect the changes in body weight and serum anti-Müllerian hormone (AMH) levels, as well as the pathological changes of the ovaries and the number of follicles at various levels. versus

　　Result: Compared with group A, the estrus cycle of rats in each treatment group was obviously disordered, the weight was significantly reduced, and the serum AMH level was also significantly reduced (Pu003c0.05). The decline in group E was the most significant, and the ovaries of each model group were different. The number of graded follicles was significantly reduced (P\u003c0.05), and the number of grades of follicles in group E and F were significantly reduced (P-0.01).

　　Conclusion: In rats, intraperitoneal injection of 4 mg/kg CP followed by weekly reinjection has a better effect on modeling POF induced by chemotherapy, and the modeling is simple.