动物造模,自发性肝硬化 z-bio 2021-06-21 347

　　Objective: To study the pathological and serum biochemical changes in the liver of Beagle dogs with spontaneous cirrhosis, and to establish background data for experimental animals suitable for GLP.

　　Methods: Blood was collected from the veins of the lower extremities to prepare serum, and the liver function indexes such as ALT, AST, TP, ALB, ALP, TBIL, TC, TG, GGT were measured with an automatic biochemical analyzer, and the diseased animals and blank control at the same time as Beagle dogs The above indicators of the animal, histopathological examination and histopathological characteristics of the liver are described under an optical microscope.

　　Results: Compared with the blank control during the same period, the ALT, AST, ALP, TBIL, GGT of the affected animals were significantly increased, and ALB was significantly decreased; liver cirrhosis showed nodular regeneration compared with normal tissues. The arrangement is chaotic, forming various pseudo-lobules, and there are proliferating collagen fibers around.

　　Conclusion: The pathological monitoring of natural lesions in beagle dogs should be strengthened to provide background information for the drug safety evaluation of laboratory animals.