动物造模,幽门螺杆菌检测 z-bio 2022-01-07 549

　　Objective: To establish a detection technique for detecting Helicobacter pylori infection in gerbils through gastric juice, and to continuously monitor Helicobacter pylori infection in gerbils using nested PCR.

　　Method: Helicobacter pylori is cultured in vitro, and gerbils are inoculated. Ten weeks after infection, the gastric juice of gerbils was detected by normal PCR. The nested PCR method was used to detect gastric juice, gastric tissue and duodenum. While checking the contents and colon feces, the accuracy of the nested PCR method was compared and analyzed by rapid urea detection and serum ELISA method. The above PCR products have been verified by sequencing.

　　Results: The conventional PCR method detects gastric juice, gastric juice, stomach tissue, duodenal contents, colon stool, rapid urease test, and nested PCR test. The results of serum ELISA test are 30~100? speed respectively. , 100?90?10?100?0? Compare the test results of different detection methods, the detection rate of gastric juice nested PCR, gastric tissue nested PCR, and rapid urease detection is 100? Routine PCR is used for gastric juice detection, colon stool Nested PCR, serological testing. The detection rate of ELISA testing method is relatively low.

　　Conclusion: The gastric juice nested PCR detection method has high specificity, accuracy and sensitivity, and can be used for Mongolian Geville long-term detection of Helicobacter pylori, especially in the prevention and treatment of Helicobacter pylori infection.