动物造模,大脑中动脉M1段栓塞模型 z-bio 2021-06-21 763

　　Objective: To provide a simple, rapid and safe method of anesthesia for large experimental animals such as rhesus monkeys that need to establish a middle cerebral artery M1 embolization model.

　　Method: Use 20 healthy adult rhesus monkeys. All males, 7-9 years old, weighing 7-11 kg, were injected intramuscularly with a mixture of 0.1 ml/kg of ketamine and silk moss for basic anesthesia, and waited for the animals to fall asleep. After tracheal intubation (ID: 4.5-5.5#). Subsequently, he was sent to the intervention center. After entering the room, he connects to the monitor and establishes venous access for complete invasive arterial pressure and catheter placement. During the operation, the experimental animals were ventilated, propofol was continuously injected at 2-4 mg/kg/h to maintain anesthesia, and the dose was adjusted according to the animal’s vital signs and intraoperative limb activity. If necessary, add the above-mentioned ketamine and fast sleep, mix it freshly, and adjust the heart rate, blood pressure, body temperature, etc. according to the needs of the operation. Brain MRI imaging was performed before and after thrombolysis. During imaging, the anesthesia injection was stopped and the animals resumed spontaneous breathing. During the operation, the heart rate, body temperature, invasive arterial blood pressure and blood oxygen saturation of the experimental animals were monitored, and the arterial blood was collected for blood gas analysis after the induction of anesthesia and during the operation.

　　Result: A total of 20 experiments were carried out, all of which met expectations. No serious complications such as irritability, respiratory depression, and arrhythmia occurred during the operation. After stopping the experiment, the experimental animals woke up immediately. I went back to animal experiments. Follow-up processing center. Of the 20 rhesus monkeys, 15 survived more than 24 hours, and 5 died of cerebral hemorrhage and large-scale cerebral infarction after thrombolysis.

　　Conclusion: Intubation combines general anesthesia with maintenance compound anesthesia, and provides a safe and practical anesthesia method for successfully completing such more complex interventional and MRI experiments.