动物造模 z-bio 2021-06-22 587

　　Objective: To explore the effect of rat serum time on the results of 14 blood biochemical tests.

　　Method: 40 adult SD rats were randomly selected, venous blood was collected on an empty stomach, left for 1 hour, serum was separated, sealed, and stored in a refrigerator at 4°C and -20°C. .Automatic biochemical analyzer, separated serum 0 hour, 4 hours, 24 hours, 96 hours, 7 days later alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), creatinine (CREA-J), uric acid (UA), urea nitrogen (UREA), blood sugar (GLU), total cholesterol (TC), triglycerides (TG), low-density lipoprotein Cholesterol (LDL)-C), lactate creatinine kinase (CK), hydrogenase (LDH) and other 14 blood biochemical indicators, and compare them.

　　Result: The change trend of blood biochemical data of male and female rats is the same. When stored at 4°C, compared with 0 hours, ALP decreased significantly at 4, 24, 96 and 7 days (P0.05). When stored at -20°C, compared with 0 hours, ALT increased significantly on the 7th day (P0.05).

　　Conclusion: Rat blood biochemical determination, especially ALP determination, should be completed as soon as possible after serum collection. When refrigerated at 4°C, the measured value of UA should not exceed 4 hours, and the measured value of ALB and CREA should not exceed -J for -J. The measurement of ALT, AST, TP, urea, GLU, TC, TG, LDL-C, CK, LDH and other indicators should not exceed 7 days.