动物造模 z-bio 2022-10-21 462

　　Objective: To improve the method of extracting rat cerebrospinal fluid based on the negative pressure suction device method, and to increase the success rate of rat cerebrospinal fluid collection?

　　Method: Do I need to add a flow regulator to control the intensity of negative pressure? Limit the amount of cerebrospinal fluid each time. The original device was modified to divide 36 male SD rats into 3 groups randomly, and the cerebrospinal fluid was extracted by percutaneous pool puncture, negative pressure suction device, and modified negative pressure suction device. ...Use the Kruskal-Wallisank Total Test to give 3 points. What is the difference between these two methods?

　　Result: The improved negative pressure aspirator method can effectively reduce bleeding, the quality of the cerebrospinal fluid obtained is higher than that of the negative pressure aspirator method (Pu003c0.01) and the success rate of collecting qualified samples is 75? The skin-cerebrospinal fluid puncture method (Pu003c0.001)?

　　Conclusion: The improved negative pressure suction device is convenient to manufacture, simple to operate, not easy to damage, and not easy to bleed.