动物造模 z-bio 2021-06-22 606

　　OBJECTIVE: To establish a safe and effective method of B-ultrasound guided in vivo puncture to obtain liver and kidney specimens in rhesus monkeys.

　　Method: Male 8-12 kg 10-12 year old macaques are anesthetized by intramuscular injection of 5-10 mg/kg ketamine. After anesthesia, place the abdomen supine on the operating table.. intercostal area. Shave the lower ventral lumbar intercostal margin and disinfect regularly with iodophor. Connect the B-superconducting rack to the 3.5Hz color computer imaging probe to explore the intercostal area of Makaku's upper right abdomen and the hip and ribs lower abdomen area. The liver or MyLab30 CVB ultrasound will further position the probe to adjust and check the target point, paying attention to avoiding the portal vein, lower abdomen, etc. For large blood vessels, adjust the range of the biopsy gun to 15mm and insert it. Under the guidance of the puncture guide, a puncture needle of about 1 cm is inserted vertically from the skin to the abdomen, the B-ultrasound image is observed in real time, and the puncture target is established. The location of the puncture needle is in the guide. Press the trigger button on the puncture gun where the puncture target is perpendicular to the puncture needle to perform puncture. Pull out the puncture needle quickly after puncture. RNA extraction was performed on the obtained liver and kidney tissues.

　　Result: The amount of liver or kidney tissue sample obtained from each puncture of each animal is approximately 13 mg after conversion. The extracted RNA has high purity and good integrity. This method is fast, reliable and safe. All experimental monkeys recovered well. after surgery. , No major bleeding or death.

　　Conclusion: This method is very important for safely and effectively obtaining ultrasound-guided liver and kidney tissue samples of rhesus monkeys. This method can reduce laboratory research costs, improve the quality of life and animal welfare of non-human primates, and at the same time ensure the accuracy of experimental results.