动物造模,EV71 z-bio 2021-06-23 826

　　Objective: To study the toxicity of different EV71 strains to newborn mice.

　　Methods: Inoculate clinically mild, severe, and death-derived EV71 strains, 1-day-old BALB/c pups, observe the symptoms of mice for 14 days, mice 1, 3, 5, and 7 and take them for 9 days. Tissue radiotherapy after infection ? Viral load was measured by QPCR, and lesions were observed by HE staining.

　　Result: There was no significant difference in the severity of symptoms between severe and mild strains (P=0 693), and both groups were heavier than dead strains (P=0). The survival rate of mice in mild, severe and mortality rates were 77-2? 81-7 97-8? The difference was significant (000, P = 0 000). P = 0.0010, P = 0 001, P = 0.0004); pulmonary hemorrhage of the mild strain is the most serious, and there is no significant difference in the pathological characteristics of brain, muscle, spleen or intestine; the proportion of the virus in each tissue of the mild strain group is the lowest, and the The highest degree, the lowest dead strain group.

　　Conclusion: Clinically, mild virus strains are the most serious in lactating mice, with few deaths. It is inconsistent with human clinical results, and may be related to viral genes, host and other factors.