动物造模 z-bio 2021-06-23 400

　　Purpose: This study investigates whether the animal nose aerosol exposure device can work normally and produce aerosol particles of a specific size, as well as the airtightness, distribution uniformity and system of the aerosol during the operation of the device, and the purpose is to explore its disinfection effect.

　　Method: Do you use an animal nasal aerosol exposure device to produce E.coli aerosol? Mouse hepatitis virus (mousehepatitisvirus) MHV? Use 75 alcohol and 3 hydrogen oxide to sterilize the JHM aerosol, respectively, the E. coli comes from the exposed compartment? MHV? JHM? E. coli collection and disinfection? MHV? JHM? Collection of cultured E. coli to collect MHV? RNA extraction from JHM, one step RT? Detection of viral RNA in PCR samples?

　　Result: The median diameter of E. coli aerosol particles produced by this device is (1.27±0.61) μm, and colonies are formed on the sample fluid coating plate and the NC in the exposure chamber. The membranes are placed at different ports of the exposure chamber. The upper layer has the same number of colonies, aseptic colonies grow on the sedimentation plate in the leakage area, and sterile colonies grow on the sterilized sampling plate. What is the MHV in the sample solution in the exposure chamber? JHM virus RNA is positive, and the virus RNA in the sampling solution is easy to leak. Negative, the viral RNA in the sample solution after disinfection is negative

　　Conclusion: The animal nose aerosol exposure device generates aerosol during normal operation. During operation, the aerosol is evenly distributed and airtight through each port of the exposure chamber. well. The corresponding disinfectant can be used to effectively disinfect the system.