动物造模 z-bio 2021-06-23 748

　　Purpose: How to investigate the bacterial transport status of rats and mice in an experimental animal production facility in Shanghai?

　　Method: Compare domestic and foreign laboratory animals and Helicobacter pylori bacteria monitoring programs? Citrobacter? Corynebacterium bovis? Staphylococcus aureus? We use pneumonia as the test item and choose Pseudomonas viridans and Klebsiella. In 2014 and 2016, 848 samples were collected respectively. Have you detected the above six pathogens by PCR?

　　Result: In 2014, SPF-level facilities were free of Staphylococcus aureus pollution, clean-level facilities were free of Klebsiella pneumoniae pollution, and other pathogens were polluted to varying degrees in facilities of different levels. All clean-level facilities are contaminated with Helicobacter pylori and Corynebacterium bovis. Helicobacter pylori? Citrobacter rodents and animals of different levels in the genus Bos Helicobacter pylori? Is the positive rate of Pseudomonas aeruginosa showing a downward trend, and the positive rate of rodent Citrobacter, Corynebacterium bovis and Klebsiella pneumoniae is on the rise?

　　Conclusion: This study conducted a comprehensive and systematic analysis of the bacterial transport status. Shanghai experimental rat and mouse study. It has promoted the improvement of the quality of laboratory animals and the level of feeding and management, and provided basis and reference for the revision and improvement of my country's national standards for laboratory animals.